human bcl2 polyclonal antibody  (Proteintech)

Journal: PLOS One

Article Title: Synergistic effects of notoginsenoside R1 and saikosaponin B2 in atherosclerosis: A novel approach targeting PI3K/AKT/mTOR pathway and macrophage autophagy

doi: 10.1371/journal.pone.0326687

Figure Lengend Snippet: (A) ox-LDL induces ROS generation in RAW264.7 cells, visualized by inverted fluorescence microscopy (scale bar = 50 μm). (B) Representative images of apoptosis detected by flow cytometry. (C) Representative Western blot images showing protein expression of BAX, BCL2, and Caspase-3 in the ox-LDL-induced cell model. β-actin was used as a loading control. (D-F) Quantification of the relative protein levels of BAX, BCL2, and Caspase-3 normalized to β-actin, and presented as relative intensity measured by Western blot analysis. All data were presented as the mean ± SD, (n = 3).** p < 0.01 vs. control group, ## p < 0.01 vs. model group, $$ p < 0.01 vs. NGR1 group, + p < 0.05, ++ p < 0.01 vs. SSB2 group.

Article Snippet: The antibodies included: AKT Monoclonal antibody (60203–2-Ig, Proteintech, China), Phospho-AKT (Ser473) Monoclonal antibody (66444–1-Ig, Proteintech, China), mTOR Monoclonal antibody (66888–1-Ig), Phospho-mTOR (Ser2448) Monoclonal antibody (67778–1-Ig, Proteintech, China), PI3 Kinase p110 Beta Polyclonal antibody (20584–1-AP, Proteintech, China), Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) Antibody (4228T, CST, USA), IL-1 Beta Polyclonal antibody (16806–1-AP, Proteintech, China), IL-18 Polyclonal antibody (10663–1-AP, Proteintech, China), Nlrp3 Polyclonal antibody (30109–1-AP, Proteintech, China), Caspase 3/p17/p19 Polyclonal antibody (19677–1-AP, Proteintech, China), BAX Polyclonal antibody (50599–2-Ig, Proteintech, China), Bcl2 Polyclonal antibody (A00040-1, Boster, China), Anti-LC3B/MAP1LC3B Antibody (BM4827, Boster, China), Beclin-1 Antibody (3738T, CST, USA), Anti-P62/SQSTM1 Antibody (M00300-1, Boster, China).After incubation with secondary antibodies (BA1056, Boster, China) for 1 hour, protein bands were visualized using an enhanced ECL chemiluminescence reagent (P0018AM, Beyotime, China) and an Odyssey infrared imaging scanner (LI-COR Biosciences).

Techniques: Fluorescence, Microscopy, Flow Cytometry, Western Blot, Expressing, Control

Journal: PLOS One

Article Title: Synergistic effects of notoginsenoside R1 and saikosaponin B2 in atherosclerosis: A novel approach targeting PI3K/AKT/mTOR pathway and macrophage autophagy

doi: 10.1371/journal.pone.0326687

Figure Lengend Snippet: Representative images of Oil Red O staining showing lipid droplets in RAW264.7 cells. Cells were examined under a light microscope (scale bar = 100 μm). (B) Representative images of cell apoptosis detected by flow cytometry. (C-F) The use of gene-specific oligonucleotide primers for the real-time PCR analysis of PPAR-γ, LXR-α, ABCA1 , and ABCG1. (G) Representative Western blot images showing protein expression levels of P-mTOR, mTOR, NLRP3, BAX, BCL2, Caspase3, P62, BECLIN1, and LC3 in ox-LDL-induced cellular models. β-actin was used as the loading control. (H-O) Quantification of relative protein levels were normalized to β-actin and were presented as relative intensity. (P) Representative confocal microscopy images demonstrating the co-localization of p-mTOR (green) and Lamp-1 (red) in RAW264.7 macrophages (scale bar = 10 μm). All data were presented as the mean ± SD, (n = 3). ** p < 0.01 vs. control group, # p < 0.05, ## p < 0.01 vs. model group, $ p < 0.05, $$ p < 0.01 vs. NS group, + p < 0.05, ++ p < 0.01 vs. NS group. Rapamycin: 0.5 μM, 3-MA: 3 mM.

Article Snippet: The antibodies included: AKT Monoclonal antibody (60203–2-Ig, Proteintech, China), Phospho-AKT (Ser473) Monoclonal antibody (66444–1-Ig, Proteintech, China), mTOR Monoclonal antibody (66888–1-Ig), Phospho-mTOR (Ser2448) Monoclonal antibody (67778–1-Ig, Proteintech, China), PI3 Kinase p110 Beta Polyclonal antibody (20584–1-AP, Proteintech, China), Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) Antibody (4228T, CST, USA), IL-1 Beta Polyclonal antibody (16806–1-AP, Proteintech, China), IL-18 Polyclonal antibody (10663–1-AP, Proteintech, China), Nlrp3 Polyclonal antibody (30109–1-AP, Proteintech, China), Caspase 3/p17/p19 Polyclonal antibody (19677–1-AP, Proteintech, China), BAX Polyclonal antibody (50599–2-Ig, Proteintech, China), Bcl2 Polyclonal antibody (A00040-1, Boster, China), Anti-LC3B/MAP1LC3B Antibody (BM4827, Boster, China), Beclin-1 Antibody (3738T, CST, USA), Anti-P62/SQSTM1 Antibody (M00300-1, Boster, China).After incubation with secondary antibodies (BA1056, Boster, China) for 1 hour, protein bands were visualized using an enhanced ECL chemiluminescence reagent (P0018AM, Beyotime, China) and an Odyssey infrared imaging scanner (LI-COR Biosciences).

Techniques: Staining, Light Microscopy, Flow Cytometry, Real-time Polymerase Chain Reaction, Western Blot, Expressing, Control, Confocal Microscopy

Journal: Molecular Medicine Reports

Article Title: Pseudolaric acid B inhibits proliferation in SW579 human thyroid squamous cell carcinoma

doi: 10.3892/mmr.2015.4418

Figure Lengend Snippet: Expression of Bcl-2 and Beclin-1, and the expression of nuclear p53 at 24 h following pseudolaric acid B treatment. (A) Expression of Bcl-2 and Beclin-1. The cells were lysed and western blot analysis was performed to detect protein expression. (B) The nuclear protein was extracted and the expression of p53 was detected. Histone served as a loading control. Data are representative of three individual experiments; n=3.

Article Snippet: Mouse anti-human LC3A/B monoclonal antibody (66139-1-Ig), rabbit anti-human Beclin 1 polyclonal antibody (11306-1-AP), rabbit anti-human B-cell lymphoma 2 (Bcl-2) polyclonal antibody (12789-1-AP) and rabbit anti-human p53 polyclonal antibody (10442-1-AP) were purchased from ProteinTech Group, Inc (ProteinTech, Chicago, IL, USA).

Techniques: Expressing, Western Blot, Control